Clinical Significance of LncRNA NNT-AS1 in the Diagnosis, Deterioration and Prognosis of Sepsis

Clinical Significance of LncRNA NNT-AS1 in the Diagnosis, Deterioration and Prognosis of Sepsis

Bo Yan,^1,2 Yifeng Jiang,³ Shijie Fu⁴ and Rong Li^1,2

¹Department of Infectious Diseases, The First People’s Hospital of Neijiang, Neijiang, China.
²Department of General Surgery (Thyroid Gland/Blood Vessel), The First People’s Hospital of Neijiang, Neijiang, China.

Sepsis is a serious infectious disease that may lead to a systemic inflammatory and life-threatening condition. Early diagnosis and treatment of sepsis is important to prevent organ failure and reduce mortality. This paper investigated the expression of long non-coding RNA NNT-AS1 (lncRNA NNT-AS1) in sepsis and its relationship the development of the disease, and revealed the clinical potential of NNT-AS1 in the diagnosis and prognosis of sepsis. Clinical data of the included individuals were collected and analyzed. Acute physiology and chronic health evaluation (APACHE) II score and sequential organ failure assessment (SOFA) score were performed based on the severity of the patient’s condition. Inflammatory factors were measured by enzyme-linked immunosorbent assay (ELISA). NNT-AS1 expression was quantified by real-time quantitative polymerase chain reaction (RT-qPCR). The ability of NNT-AS1 to identify sepsis was evaluated using receiver operating characteristic (ROC) curve. The prognostic value of NNT-AS1 was confirmed by the Kaplan-Meier method and Cox regression analysis. Biochemical indicators, inflammatory factors, APACHE II score and SOFA score were higher in sepsis patients than in healthy individuals. Serum NNT-AS1 was actively expressed in sepsis and had a high diagnostic value. NNT-AS1 levels were positively correlated with APACHEII score, SOFA score, and inflammatory factor levels. Meanwhile, high expression of NNT-AS1 predicted shorter survival of patients. NNT-AS1 was highly expressed in sepsis and was closely related to the development of patients’ conditions. By monitoring the changes of NNT-AS1 may be of key value for the diagnosis, prognosis and therapeutic prognosis of patients.

Key words —— diagnosis; inflammatory factor; lncRNA NNT-AS1; prognosis; sepsis

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Tohoku J. Exp. Med., 2025 January, 265(1), 21-27.

Correspondence: Jianyuan Huang, Department of General Surgery (Thyroid Gland/Blood Vessel), The First People’s Hospital of Neijiang, No. 1866, West Section of Han’an Avenue, Neijiang, 641099, China.

e-mail:huangjianyuan7388@163.com