LncRNA SNHG14 Served as a Biomarker of Depression Disorder Patients and Regulated Depression-Like Behaviors via MiR-200a-3p

LncRNA SNHG14 Served as a Biomarker of Depression Disorder Patients and Regulated Depression-Like Behaviors via MiR-200a-3p

HongLi Wang,¹ SiWen Deng² and Juan Bi¹

¹Department of Neurology, People's Hospital of Dongxihu District, Wuhan, Hubei, China
²Department of Urology, People's Hospital of Dongxihu District, Wuhan, Hubei, China

Depression disorder has become a major mental disease and has attracted special attention globally. Identifying specific biomarkers for the diagnosis and severity of depression disorder would benefit its clinical management. This study focused on the significance of lncRNA SNHG14 in depression disorder and investigated its effect on depression-like behaviors, aiming to explore a potential biomarker for depression disorder occurrence and development. This study included 147 patients with depression disorder and 98 healthy individuals. The serum SNHG14 in all participants was analyzed by PCR, and its diagnostic value was evaluated by receiver operatorating characteristic curve (ROC) analysis. The depression-like behaviors were induced via chronic social defeat stress (CSDS) and evaluated by sucrose preference, forced swimming, and open field tests. SNHG14 was significantly upregulated in depression disorder patients relative to healthy individuals, which discriminated depression disorder patients with a relatively high efficiency. Depression disorder patients with severe conditions showed higher serum SNHG14 levels, and a significantly positive correlation of SNHG14 with PHQ9 score was demonstrated. In CSDS mice, increasing SNHG14 and decreasing miR-200a-3p were observed. Silencing SNHG14 and overexpressing miR-200a-3p could alleviate reduced sucrose preference, increased swimming immobility time, decreased standing times, and decreased traveling distance induced by CSDS. The knockdown of SNHG14 promoted the expression of miR-200a-3p, and silencing miR-200a-3p could reverse the protective effect of SNHG14 silencing on depression-like behaviors. SNHG14 served as a biomarker for the occurrence and severity of depression disorder. Silencing SNHG14 could alleviate depression-like behaviors via modulating miR-200a-3p.

Key words —— anhedonia; autonomy and exploratory behaviors; despairing behaviors; diagnosis; PHQ9 score

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Tohoku J. Exp. Med., 2024 May, 263(1), 55-62.

Correspondence: HongLi Wang, Department of Neurology, People's Hospital of Dongxihu District, No.48, Jinbei 1st Road, Jinghe Street, Dongxihu District, Wuhan, Hubei 430040, China.

e-mail: WangHongLi_WH@163.com