LncRNA TRHDE-AS1 and MiR-1275 as Promising Prognostic Biomarkers and Effects on Tumor Cell Progression in Gastric Cancer

LncRNA TRHDE-AS1 and MiR-1275 as Promising Prognostic Biomarkers and Effects on Tumor Cell Progression in Gastric Cancer

Fengli Liu,¹ Jiyan Cui¹ and Dongyun Xue¹

¹Department of Gastroenterology, Shandong Provincial Third Hospital, Shandong University, Jinan, Shandong, China

Gastric cancer is a common malignant tumor with a relatively poor prognosis after surgery. Non-coding RNA may serve as biomarkers for the progression and prognosis of various cancers. The clinical significance and biological function of lncRNA TRHDE-AS1 and miR-1275 in gastric cancer were assessed in this study. 119 paired tissues were selected with adequate clinical information. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to determine the expression level of lncRNA TRHDE-AS1 and miR-1275 in gastric cancer tissues and cells. The association between lncRNA TRHDE-AS1 or miR-1275 expression and the clinicopathological features of patients was analyzed by the Pearson Chi-square test. Kaplan-Meier analysis and Multi-variate Cox proportional regression analysis were utilized to evaluate the prognostic value of lncRNA TRHDE-AS1 and miR-1275. Finally, the effect of TRHDE-AS1 binding to miR-1275 on the gastric cancer cellular process was investigated by CCK-8 and Transwell assay. LncRNA TRHDE-AS1 was found to be downregulated in gastric cancer tissues and cells, but miR-1275 upregulated, which both showed significant associations with clinical pathology of gastric cancer patients (including TNM stage and lymph node metastasis) and a poor prognosis. LncRNA TRHDE-AS1 and miR-1275 can be considered two independent prognostic factors for gastric cancer. Furthermore, the upregulation of lncRNA TRHDE-AS1 inhibited cell proliferation, migration, and invasion of gastric cancer partly by miR-1275. LncRNA TRHDE-AS1/miR-1275 axis may be involved in the progression of gastric cancer and can be promising prognostic factors, which may provide new insights into the treatment of gastric cancer.

Key words —— gastric cancer; miR-1275; prognosis; progression; TRHDE-AS1

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Tohoku J. Exp. Med., 2023 September, 261(1), 13-23.

*These two authors contributed equally to this work.

Correspondence: Dongyun Xue, Department of Gastroenterology, Shandong Provincial Third Hospital, Shandong University, No. 11 Wuyingshan Middle Road, Tianqiao District, Jinan, Shandong 250031, China.

e-mail: xuedongyun_1981@163.com