Tohoku J. Exp. Med., 2022 June, 257(2)

RECK Variants are Associated with Clinicopathological Features and Decreased Susceptibility in Mexican Patients with Colorectal Cancer

Rosa María Márquez-González,1 Anilú Margarita Saucedo-Sariñana,1 Patricio Barros-Núñez,2,3 Martha Patricia Gallegos-Arreola,4 Clara Ibet Juárez-Vázquez,5 Tomás Daniel Pineda-Razo,6 María Eugenia Marin-Contreras,7 Silvia Esperanza Flores-Martínez1,3 and Mónica Alejandra Rosales-Reynoso1

1Molecular Medicine Division, Centro de Investigación Biomédica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, México
2Research Unit of Metabolic Diseases, Pediatric UMAE, Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco. México
3Doctorado en Genética Humana, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, México
4Genetic Division, Centro de Investigación Biomédica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, México
5Academic Direction of Devices and Systems I, Facultad de Medicina, Decanato Ciencias de la Salud, Universidad Autónoma de Guadalajara (UAG), Zapopan, Jalisco, México
6Medical Oncology Service, Specialty Hospital, Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, México
7Gastroenterology Service, Specialty Hospital, Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, México

Colorectal cancer (CRC) is the third most common cancer and the second leading cause of death worldwide. Down-regulation of the cysteine-rich reversion-inducing protein with Kazal motifs (RECK) has been confirmed in numerous human cancers and is clinically associated with metastasis. This study aims to explore, for the first time, the possible association of the RECK variants rs11788747 and rs10972727 with CRC susceptibility and clinicopathological features. DNA from 130 CRC patients and 130 healthy blood donors was analyzed. Identification of genetic variants was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methodology. Association was calculated using the odds ratio (OR) test and P values were adjusted using the Bonferroni test. Individuals carrying the G/G genotype for the rs11788747 variant showed a lower risk of colorectal cancer (OR 0.33; 95% CI 0.16-0.70; P = 0.006). Patients older than 50 years who carry the G/G genotype have a lower risk of CRC (OR 0.26; 95% CI 0.09-0.73; P = 0.019) and of developing advanced tumor-nodule-metastasis (TNM) stages (OR 0.23; 95% CI 0.09-0.54; P = 0.001). Individuals carrying the A/A genotype of the rs10972727 variant also showed decreased risk of CRC (OR 0.38; 95% CI 0.19-0.77; P = 0.011), and were associated with age (over 50 years), sex, advanced TNM stages, and tumor location in the colon. Our results suggest that the RECK variants studied here (rs11788747 and rs10972727) are associated with decreased CRC risk, TNM stages and tumor location.

Keywords —— colorectal cancer; decreased susceptibility; haplotypes; RECK variants; TNM stage; tumor location

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Tohoku J. Exp. Med 2022, 257, 163-169.

Correspondence: Mónica Alejandra Rosales-Reynoso, Ph.D., División de Medicina Molecular, Centro de Investigación Biomédica de Occidente (CIBO), Centro Médico Nacional de Occidente (CMNO), Instituto Mexicano del Seguro Social (IMSS), Sierra Mojada #800, Colonia Independencia, Guadalajara, Jalisco CP 44340, México.

e-mail: mareynoso77@yahoo.com.mx; monica.rosales@imss.gob.mx