Insights from postmortem brain analysis by sandwich ELISAs

Mitsuru Shinohara

Section Chief of the Molecular Neuropharmacology, Department of Aging Neurobiology,
Center for Development of Advanced Medicine for Dementia, National Center for Geriatrics and Gerontology

To develop effective treatments for Alzheimer’s disease （AD）, further understanding of the disease pathology is necessary, including how amyloid-beta （Aβ）, tau and apolipoprotein E （apoE） accumulates and interact with each other in the brain.　By using sandwich ELISAs and focusing on region-specific accumulation of Aβ, we have analyzed postmortem frozen brains of many subjects, including non-demented individuals, sporadic AD, familiar AD, and AD with/without APOE ε4 genotype.　We have obtained novel observations regarding their accumulations, which would provide important insights into the pathogenesis of AD.　Here, we review our research results together with highlighting the utility of sandwich ELISAs.

Address correspondence to Dr. Mitsuru Shinohara, Section Chief of the Molecular Neuropharmacology, Department of Aging Neurobiology, Center for Development of Advanced Medicine for Dementia, National Center for Geriatrics and Gerontology （7-430 Morioka-cho, Obu City, Aichi 474-8511, Japan）