CircRNA CircABCB1 Diminishes the Sensitivity of Breast Cancer Cells to Docetaxel by Sponging MiR153-3p

CircRNA CircABCB1 Diminishes the Sensitivity of Breast Cancer Cells to Docetaxel by Sponging MiR153-3p

Jiaqi Liu,¹ Linna Kong,¹ Wenqing Bian,² Xiaona Lin,¹ Feifei Wei³ and Jun Chu¹

¹Department of Breast and Thyroid Surgery, Zibo Central Hospital, Zibo, Shandong, China
²Maternal and Child Health Hospital of Zibo, Zibo, Shandong, China
³Weifang Maternal and Child Health Hospital, Weifang, Shandong, China

Resistance to docetaxel is a major problem to the success of docetaxel-based therapies for breast cancer. The present study was to identify the role of circABCB1 in altering the docetaxel resistance properties. Reverse transcriptionquantitative PCR (qRT-PCR) was performed to quantify circABCB1 and miR-153-3p. The effects of circABCB1 on the viability, apoptosis and migration/invasion of docetaxel-resistant and -sensitive cells were investigated by cell function experiments, including Cell Counting Kit8 and Transwell assays. Correlation between circABCB1 and the docetaxel-treated outcome was analyzed by multivariate Cox regression analysis, in addition to Kaplan-Meier analysis of time to treatment failure (TTF). The targeting relationship between circABCB1 and miR153-3p was predicted and verified by dualluciferase reporter assay and RNA immunoprecipitation. CircABCB1 was highly expressed in cancerous tissues, as well as the docetaxel-sensitive group and cells. The overexpression of circABCB1 contributed to cell viability, docetaxelresistance and migration/invasion, but inhibited apoptosis. CircABCB1 can sponge miR153-3p. CircABCB1 contributed to the docetaxel resistance of breast cancer, maybe via the miR153-3p.

Key words —— breast cancer; ceRNA; circABCB1; docetaxel resistance; prognosis

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Tohoku J. Exp. Med., 2023 September, 261(1), 25-33.

Correspondence: Jun Chu, Department of Breast and Thyroid Surgery, Zibo Central Hospital, 54 Gongqingtuan West Road, Zibo, Shandong 255020, China.

e-mail: junchu_dr@163.com