Sleep Disturbance and Hyperactivity Detected by Actigraphy in Rats with Allergic Rhinitis or Attention-Deficit Hyperactivity Disorder

Sleep Disturbance and Hyperactivity Detected by Actigraphy in Rats with Allergic Rhinitis or Attention-Deficit Hyperactivity Disorder

MOTOHIKO SUZUKI,¹ MEIHO NAKAYAMA,^1,2 KAYOKO BHARDWAJ ANDO,² SACHIE ARIMA,^1,2 YOSHIHISA NAKAMURA,¹ MAKOTO YOKOTA¹ and SHINGO MURAKAMI¹

¹Department of Otorhinolaryngology, Nagoya City University, Nagoya, Aichi, Japan
²Good Sleep Center, Nagoya City University Hospital, Nagoya, Aichi, Japan

Actigraphy is an easy and noninvasive method used to monitor human ultradian cycles. However, to our knowledge, it has been not applied to experiments with rodents. Therefore, using actigraphy, we assessed the ultradian cycles and behavior of rats. Rats with or without allergic rhinitis wore an actigraphy device, and triaxial acceleration was recorded. The counts that represent physical activity were lower from 8:00 to 20:00 than those from 20:00 to 8:00 in control rats, suggesting that their sleep phase was from 8:00 to 20:00 and their awake phase from 20:00 to 8:00. The counts from 8:00 to 10:00 were significantly higher in allergic rhinitis rats than in control rats (p < 0.01), suggesting the presence of difficulty with sleep induction in rats with allergic rhinitis. The counts from 18:00 to 20:00 were also significantly higher in allergic rhinitis rats than in control rats (p < 0.05), suggesting the presence of early awakening in rats with allergic rhinitis. Moreover, the counts were significantly higher in allergic rhinitis rats than in control rats from 20:00 to 8:00. These results suggest that rats with allergic rhinitis experienced hyperactivity disorder during the daytime. Additionally, hyperreactivity and difficulty with sleep induction were observed in 6-hydroxydopamine-lesioned rats, an animal model of attention-deficit hyperactivity disorder. This study shows for the first time that actigraphy can be successfully used for behavioral analysis in rodents. These rat models could be useful for analyzing the mechanisms involved in sleep disturbances and hyperactivity disorder.

keywords —— actigraphy; allergic rhinitis; hyperactivity; rat; sleep disturbance

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Tohoku J. Exp. Med., 2018, 246, 65-71

Correspondence: Motohiko Suzuki, M.D., Ph.D., Departments of Otorhinolaryngology, Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan.

e-mail: suzu-mo@med.nagoya-cu.ac.jp