Tohoku J. Exp. Med., 2017 November, 243(3)

Elevated Expression Levels of Long Non-Coding RNA, Loc554202, Are Predictive of Poor Prognosis in Cervical Cancer

JIEWEN CHEN¹ and JIE ZHU²

¹Center for Reproductive Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University and Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai, China
²Department of Obstetrics & Gynecology, Shanghai Jiaotong University Affiliated Renji Hospital, Shanghai, China

Cervical cancer remains one of the most common causes of gynecological cancer-associated death. Long non-coding RNA Loc554202 (lncRNA Loc554202) has been reported to be involved in the development of several types of cancer. However, the role of Loc554202 in cervical cancer remains unclear. In this study, we measured the expression levels of Loc554202 in cervical cancer tissues from 120 patients. The quantitative real-time PCR analysis showed the expression levels of Loc554202 were significantly higher in cervical cancer tissues compared with the adjacent non-tumor tissues. Elevated expression levels of Loc554202 were significantly associated with tumor size (p = 0.006), FIGO stage (p = 0.015), HPV (p = 0.001), and lymph node metastasis (p = 0.002). Kaplan-Meier analysis clearly illustrated that patients with high expression levels of Loc554202 had a lower overall survival rate compared to patients with lower expression (p = 0.0013). Furthermore, we show that Loc554202 is an independent poor prognostic factor through multivariate analysis. Subsequently, using cervical cancer cell lines, HeLa and ME-180, we decreased the expression levels of Loc554202 with siRNA. As results, the proliferation ability of cervical cancer cells was inhibited and apoptosis was induced after Loc554202 knockdown, as judged by viability assay, colony formation, and flow cytometry. Moreover, knockdown of Loc554202 expression down-regulated Bcl-2 expression and conversely up-regulated Bax expression in cervical cancer cells using Western blotting analysis. In conclusion, elevated levels of Loc554202 are predictive of poor prognosis in cervical cancer. We suggest that Loc554202 may serve as a potential therapeutic target for cervical cancer.

keywords —— cervical cancer; long non-coding RNA; Loc554202; prognosis; proliferation

© 2017 Tohoku University Medical Press

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Tohoku J. Exp. Med., 2017, 243, 165-172

Correspondence: Jie Zhu, Department of Obstetrics & Gynecology, Shanghai Jiao Tong University School of Medicine Affiliated Renji Hospital, NO.160 Pujian Road, Pudong New Area, Shanghai 200135, China.

e-mail: jieZZhu_2016@163.com