Tohoku J. Exp. Med., 2016 September, 240(1)

The Polymorphism rs3024505 (C/T) Downstream of the IL10 Gene Is Associated with Crohn's Disease in Serbian Patients with Inflammatory Bowel Disease

DRAGANA MIJAC,¹ IRENA VUKOVIC PETROVIC,² SRDJAN DJURANOVIC,¹ VLADIMIR PEROVIC,² DANIELA BOJIC,³ DJORDJE CULAFIC,¹ DRAGAN POPOVIC,¹ MIODRAG KRSTIC,¹ GORAN JANKOVIC,¹ MILICA DJORIC,² VERA PRAVICA² and MILOS MARKOVIC²

¹Clinic for Gastroenterology and Hepatology, Clinical Center of Serbia, School of Medicine, University of Belgrade, Belgrade, Serbia
²Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Belgrade, Serbia
³University Hospital Zvezdara, Department of Gastroenterology, School of Medicine, University of Belgrade, Belgrade, Serbia

Inflammatory bowel disease (IBD), manifesting as Crohn's disease (CD) and ulcerative colitis (UC), is characterized by recurring episodes of inflammation in gastrointestinal tract, in which aberrant production of regulatory cytokine interleukin-10 (IL-10) presumably plays important role. Single nucleotide polymorphisms (SNPs) that affect IL-10 production, such as rs1800896 (G/A) at position -1082 and rs1800871 (C/T) at position -819 in the promoter region of the IL10 gene, have been associated with CD and/or UC, but the results were inconsistent. Another SNP that may alter IL-10 production, rs3024505 (C/T) located immediately downstream of the IL10 gene has been recently identified. T allele of rs3024505 was associated with both UC and CD in Western populations, but the studies from East European countries are lacking. Therefore, our aim was to assess the association of rs3024505, rs1800896 and rs1800871 with Serbian IBD patients. To this end, 107 CD and 99 UC patients and 255 healthy controls were genotyped. As a result, T allele of rs3024505 was associated with CD at allelic, genotypic (GT genotype) and haplotypic (GCCT haplotype) level, suggesting potential role of this variant in susceptibility to CD. In contrast, CD patients carrying C allele of rs3024505 had significantly increased risk of anemia and stricturing/penetrating behavior. No association was observed between rs3024505 and UC or SNPs in IL10 promoter region and any form of IBD. In conclusion, rs3024505 SNP flanking the IL10 gene is associated with susceptibility and severity of disease in Serbian CD patients, further validating its role as a potential biomarker in IBD.

keywords —— Crohn's disease; interleukin-10; rs3024505; single nucleotide polymorphisms; ulcerative colitis

© 2016 Tohoku University Medical Press

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Tohoku J. Exp. Med., 2016, 240, 15-24

Correspondence: Dragana Mijac, M.D., M.Sc., Clinic for Gastroenterology and Hepatology, Clinical Center of Serbia, School of Medicine, University of Belgrade, Dr Koste Todorovica 2, Belgrade 11 000, Serbia.

e-mail: draganamijac@gmail.com