Tohoku J. Exp. Med., 2015 June, 236(2)

Elevated Serum Levels of Heat Shock Protein 70 Are Associated with Breast Cancer

MERAL GUNALDI,¹ CIGDEM USUL AFSAR,² YILDIZ OKUTURLAR,³ ASUMAN GEDIKBASI,⁴ HAKAN KOCOGLU,³ ALEV KURAL,⁴ CEVHER AKARSU,⁵ UMUT GUNDUZ⁶ and ELIF EDA TIKEN⁷

¹Department of Medical Oncology, Bakirkoy Dr. Sadi Konuk Education and Research Hospital, Istanbul, Turkey
²Department of Medical Oncology, Istanbul Education and Research Hospital, Istanbul, Turkey
³Department of Internal Medicine, Bakirkoy Dr. Sadi Konuk Education and Research Hospital, Istanbul, Turkey
⁴Department of Biochemistry, Bakirkoy Dr. Sadi Konuk Education and Research Hospital, Istanbul, Turkey
⁵Department of General Surgery, Bakirkoy Dr. Sadi Konuk Education and Research Hospital, Antalya, Turkey
⁶Department of General Surgery, Antalya Education and Research Hospital, Antalya, Turkey
⁷Department of Radiation Oncology, Bakirkoy Dr. Sadi Konuk Education and Research Hospital, Istanbul, Turkey

Breast cancer (BC) is the most frequent cause of cancer death in women throughout the world. Thus, it is necessary to establish sensitive screening, diagnosis and treatment methods for BC. Heat shock protein 70 (HSP70) is an important cellular stress response protein that protects cells from apoptosis. Recent studies have shown that serum HSP70 levels may provide clinically important information in various types of cancer. HSP70 is also overexpressed in BC, which is known to be associated with cancer progression, apoptosis and cell proliferation. However, the serum level of HSP70 and its diagnostic and prognostic potential in BC have not been investigated yet. The aim of this study was to determine the usefulness of serum HSP70 level as a diagnostic test and its predictive value in patients with BC. This prospective study consisted of 45 female patients diagnosed with BC and 16 healthy women who were matched for age and body mass index (BMI). Enzyme-linked immunosorbent assay (ELISA) technique was used to measure the serum level of HSP70. The serum level of HSP70 was significantly higher in patients with BC than in the healthy control group (5.98 ± 2.05 vs. 1.49 ± 0.47 ng/ml, p = 0.001). HSP70 level > 2.41 ng/ml was the best cutoff value to predict BC (97.78% sensitivity and 93.75% specificity). This study shows that HSP70 can be used as an adjunct to other diagnostic tests for BC and may be helpful for identifying patients at increased risk of BC.

keywords —— breast cancer; cut-off value; diagnosis; heat shock protein 70; target therapy

© 2015 Tohoku University Medical Press

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Tohoku J. Exp. Med., 2015, 236, 97-102

Correspondence: Meral Gunaldi, Department of Medical Oncology, Bakirkoy Dr. Sadi Konuk Education and Research Hospital, Zuhuratbaba district, Tevfik Saglam Street, No. 11, 34147 Bakirköy, Istanbul, Turkey.

e-mail: meralgunaldi@gmail.com