Tohoku J. Exp. Med., 2011, 224(2)

Haplotype-Based Association of Four Lymphotoxin-Alpha Gene Polymorphisms with the Risk of Coronary Artery Disease in Han Chinese

YAN LIU,¹ HAIHUI SHENG,² LIN LU,¹ ZHIJUN WU,¹ QIUJIN CHEN,¹ HUASHENG XIAO² and WEI JIN¹

¹Department of Cardiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, P.R. China
²National Engineering Center for Biochip at Shanghai, Shanghai, P.R. China

Lymphotoxin-alpha (LTA), a pro-inflammatory cytokine, has been implicated in the pathogenesis of coronary atherosclerosis. Meanwhile, association of some single nucleotide polymorphisms (SNPs) of LTA gene with coronary artery disease (CAD) has been evaluated; however, the results are irreproducible. We therefore investigated the relationship between four SNPs of LTA gene and CAD in Han Chinese: G+10A (rs1800683, 5'-untranslated region), A+80C (rs2239704, 5'-untranslated region), T+496C (Cys13Arg, rs2229094, exon 2), and C+804A (Thr26Asn, rs1041981, exon 3). Genotyping was performed in 438 CAD patients and 330 healthy controls. Single-locus analysis showed that the genotype and allele frequencies of G+10A polymorphism exhibited marginal differences between CAD patients and controls, although no statistical significance was observed after the Bonferroni correction. Logistic regression analysis revealed that GG genotype of G+10A polymorphism was significantly associated with the risk of CAD under the dominant mode, whereas no significant association was detected between A+80C polymorphism and CAD. In contrast, individuals carrying TT or TC genotype of T+496C polymorphism showed a decreased CAD risk relative to those with CC genotype under the recessive mode. Likewise, CC genotype of C+804A polymorphism was associated with a protective effect on CAD under the dominant mode. Further, in haplotype analysis, the haplotype G-C-T-C (in order of rs1800683, rs2239704, rs2229094 and rs1041981) was significantly associated with a decreased risk of CAD after assigning the most common haplotype A-C-T-A as a reference. In conclusion, we show a protective effect of the haplotype G-C-T-C on the occurrence of CAD, suggesting the involvement of LTA in CAD pathogenesis.

keywords —— coronary artery disease; gene; haplotype; lymphotoxin-alpha; polymorphism

© 2011 Tohoku University Medical Press

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Tohoku J. Exp. Med., 2011, 224, 119-125

Correspondence: Wei Jin, M.D., PH.D., Department of Cardiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 197 Ruijin Er Road, Shanghai 200025, P.R. China.

e-mail: jinwei_ivy@126.com