Tohoku J. Exp. Med., 2010, 220(1)
Establishing A Model of Supratentorial Hemorrhage in the Piglet
YUANHONG SHI,1,2 ZAIWANG LI,1 SUMING ZHANG,1 MINJIE XIE,1 XIANGWU MENG,1 JINZHI XU,1 NA LIU1 and ZHOUPING TANG1
1Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P.R. China
2Department of Neurology, Affiliated Hospital of Jiujiang Uinversity, P.R. China
The most common site of hemorrhage is the basal ganglia, which exhibits the obvious neurological deficits. In the present study, we aimed to develop a model of supratentorial intracerebral hemorrhage (ICH) with neurological deficits in piglets (6.0 to 8.8 kg). A pediatric urinary catheter with two passages and one balloon was introduced through a burr hole into the right striatum. All the animals received balloon inflation, which was performed by injecting 2.5 ml saline into the balloon through one passage. Then each piglet in experimental group (n = 18) received an injection of 1.0-ml autologous arterial blood through the other passage over 2 min and maintained for 5 min. Then, additional 1.5-ml blood was injected over 15 min. Piglets in control group (n = 6) received only balloon inflation without blood injection. CT scanning was performed immediately after surgery. A deep hematoma was successfully induced in 16 out of 18 piglets and the hematoma volume was 1.74 ± 0.22 ml (n = 5) at 24 hours after surgery. All the piglets with hematoma had behavioral deficits (lame or could not walk) at 24 hours. Tissue damages, such as cell swelling, necrosis and demyelization, appeared at 24 hours in the brain tissues, adjacent to the hematoma, and was aggravated at 48 hours and ameliorated at 7 days after hematoma induction. In conclusion, we have established a simple model of supratentorial ICH in piglets with marked neurological deficits, which is suitable for study of the pathophysiology and treatment of ICH.
keywords —— intracerebral hemorrhage; animal model; behaviorl; histopathology
© 2010 Tohoku University Medical Press
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Tohoku J. Exp. Med., 2010, 220, 33-40
Correspondence: Zhouping Tang, M.D., Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095, Jie-Fang-Da-Dao Wuhan, 430030, P.R. China.
e-mail: ddjtzp@163.com.