Protective Action of D-Ribose against Renal Injury Caused by Ischemia and Reperfusion in Rats with Transient Hyperglycemia

Protective Action of D-Ribose against Renal Injury Caused by Ischemia and Reperfusion in Rats with Transient Hyperglycemia

JUNJI NISHIYAMA,¹ MASAAKI UEKI,^1,2 TAKEHIKO ASAGA,² KOUSUKE CHUJO² and NOBUHIRO MAEKAWA¹

¹Department of Anesthesia and Perioperative Medicine, Kobe University Hospital Anesthesiology, Kobe, Japan
²Department of Anesthesiology, Faculty of Medicine, Kagawa University, Kagawa, Japan

Hyperglycemia amplifies the inflammatory state after ischemia/reperfusion (I/R), and activated neutrophils have been implicated in the development of I/R-induced renal injuries. D-ribose is a naturally occurring monosaccharide found in all living cells. In this study, we examined whether D-ribose attenuates I/R-induced renal injury by reducing neutrophil activation in rats with transient hyperglycemia. Male Wistar rats were divided into sham (n = 24), control (n = 64), and D-ribose (n = 32) groups. Rats received intraperitoneal injection of glucose (3 g/kg) 30 min before induction of ischemia to induce transient hyperglycemia. Anesthetized rats underwent right nephrectomy and subsequent occlusion of the left renal artery and vein for 45 min. D-ribose (400 mg/kg) was intravenously administered 30 min before induction of ischemia. D-ribose significantly reduced the degree of the I/R-induced increases in renal concentrations of cytokine-induced neutrophil chemoattractant-1 (a chemotactic factor for the activation of neutrophils and chemotaxis to the site of injury) and myeloperoxidase (an indicator of neutrophils infiltration). D-ribose also reduced the I/R-induced increases in serum levels of blood urea nitrogen and creatinine, and improved histological changes, including acute tubular necrosis in the corticomedullary junction fields. These results indicate that D-ribose reduces the I/R-induced acute renal injury in rats with transient hyperglycemia, probably by reducing neutrophil activation. D-ribose might thus be useful for surgical procedures, such as renal transplant surgery, under hyperglycemia.

keywords —— D-ribose; hyperglycemia; neutrophil; kidney; ischemia/reperfusion.

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Tohoku J. Exp. Med., 2009, 219, 215-222

Correspondence: Masaaki Ueki, Department of Anesthesia and Perioperative Medicine, Kobe University Hospital Anesthesiology, Kusunoki-cho 7, Chuo-ku, Kobe 650-0017, Japan.

e-mail: mueki@med.kobe-u.ac.jp