Tohoku J. Exp. Med., 2006, 208(4)

Inhibition of Oct4 Expression in Mouse Preimplantation Embryos Using Morpholino Antisense Oligonucleotides

ISAO TSUJI, TASUKU MITANI,^1,2 AKIYO MITSUHASHI, YO WATANABE, YOSHIHIKO HOSOI^1,3 and HIROSHI HOSHIAI

Department of Obstetrics and Gynecology, Kinki University of Medicine, Osaka, Japan, ¹Institute of Advanced Technology, Kinki University and ²Gene Control Corporation, Wakayama, Japan, and ³Department of Biology-Oriented Science and Technology, Kinki University, Wakayama, Japan

Morpholino oligonucleotides (MO) can induce gene silencing by binding to a target mRNA and inhibiting its translation, and this technique has been especially successful in studies of embryonic development in various vertebrates. But in mice MO-induced downregulation of target genes has not been widely reported. In this study, we examined whether MO delivery using ethoxylated polyethylenimine (EPEI) delivery reagent is useful for silencing gene expression in the mouse preimplantation embryo, by targeting endogenous gene Oct4. To optimize the conditions for MO delivery, we examined the MO concentration, the EPEI concentration, the treatment time, and the number of MO treatments. The MO treatment was performed at the 2-cell, the morula, the blastocyst, and the hatched blastocyst stage. We first determined the optimal conditions for MO delivery into the nucleus using fluorescein isothiocianate (FITC)-labeled MO, and demonstrated that treatment with a combination of 20 μM MO and 0.56 μM EPEI for 3 hrs produced effective MO delivery. MO-induced downregulation of Oct4 was then examined. Two-step MO treatment at the 2-cell and blastocyst stages successfully suppressed Oct4 expression. This MO treatment resulted in marked reduction of Oct4 protein at the blastocyst stage. After cultivation of blastocysts for further 4 days, derivatives of embryos either differentiated to trophoblastic cells or showed developmental arrest at the blastocyst. This phenocopy is similar to Oct4-deficient embryos. Overall, our results indicate that MO delivery with EPEI is an effective tool for analyzing gene function in mouse preimplantation embryos.

keywords —— morpholino; antisense; EPEI; blastocyst; Oct4

© 2006 Tohoku University Medical Press

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Tohoku J. Exp. Med., 2006, 208, 333-342

Correspondence: Isao Tsuji, Department of Obstetrics and Gynecology, Kinki University of Medicine, 377-2 Onohigashi, Osaka-Sayama, Osaka 589-8511, Japan.

e-mail : Issa.and.ryoma@m2.dion.ne.jp