Tohoku J. Exp. Med., 2004, 204(1)

Activation of Eosinophils by Rice-Husk Dust Exposure: A Possible Mechanism for the Aggravation of Asthma during Rice Harvest

HIROYUKI KAYABA, HITOMI MEGURO,1 HAJIME MUTO,2 YUMIKO KAMADA, TETSUYA ADACHI, YOSHIYUKI YAMADA, AKIRA KANDA, KAZUTOSHI YAMAGUCHI, KAZUYUKI HAMADA, SHIGEHARU UEKI and JUNICHI CHIHARA

Department of Clinical and Laboratory Medicine, 1Division of Central Research Laboratory, and 2Environmental Research Center, Akita University School of Medicine, Akita 010-8543

Grain dust and other irritants affect the airway of allergic patients in rice-growing area during the harvest. The aim of this study was to elucidate the mechanism of airway hypersensitivity in rice-growing areas during the harvest. Firstly, the effect of rice-husk dust on eosinophil activation was studied. Secondary, the concentration of lipopolysaccharides (LPS), a potent activator of inflammatory cells, in rice-husk dust was measured. Since it is possible for LPS, a component of gram-negative bacterial cell wall, to adhere to the particle of smoke generated from rice-husk dust, LPS contained in the smoke was also measured. Furthermore, chemical irritants contained in the smoke generated from the rice-husk dust were analyzed. Microscopically, the dust contained fine thorns dropped off from the outer sheath of the rice, and irritated the skin, throat and eyes. The grain dust extract increased the expressions of eosinophil activation markers. These up-regulatory effects were largely dependent on LPS. The smoke contained LPS and several chemical irritants such as formaldehyde and acetaldehyde. Rice-husk dust and its smoke, hazardous air pollutants, probably play a major role in the aggravation of airway diseases in agricultural areas.

keywords —— LPS; smoke; formaldehyde; asthma; rice-husk

© 2004 Tohoku University Medical Press

===============================

Tohoku J. Exp. Med., 2004, 204, 27-36

Address for reprints: Dr. Hiroyuki Kayaba, Central Clinical Laboratory, Akita University Hospital, 1-1-1 Hondo, Akita 010-8543, Japan.

e-mail: kayaba@hos.akita-u.ac.jp