Effects of Granulocyte-Colony Stimulating Factor on Wound Healing in a Mouse Model of Burn Trauma
EROL EROGLU, FATIH AGALAR, IRFAN ALTUNTAS1 and FUSUN EROGLU2
Department of General Surgery, 1Department of Biochemistry, and 2Department Anaesthesiology, Suleyman Demirel University, School of Medicine, Isparta, Turkey
The effects of burn trauma and granulocyte-colony stimulating factor (G-CSF) treatment on wound healing in a surgical incision model were studied. Sixty adult male mice were used in this study. Under general anesthesia hot water at 97°C was applied for 3 sec to the dorsum of the mice in order to achieve 20% burn wound. After burn trauma, full thickness midline skin incision 2 cm in length was performed on the abdominal wall and then were sutured primarily with 4/0 polypropylene. In Group I only skin incision was performed, group II had skin incision and burn, in group III G-CSF (0.03 BU/30 g) was applied intraperitoneally after burn and skin incision. Breaking strength and 5-hydroxyproline (5-HP) levels of the wounds were calculated 5 and 10 days after the procedure. 5-HP levels and breaking strength values showed statistical difference between groups II-III and I-II (p<0.05). 5-HP levels were lowest in incision and burn group (41.80 μg/mg). Breaking strength levels were also lowest in the same group (0.12 kg) (p<0.05). These results suggest that third degree burn causes a significant impairment on incisional wound healing and G-CSF ameliorates this impairment.
keywords —— burn; wound; wound healing; G-CSF
© 2004 Tohoku University Medical Press
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Tohoku J. Exp. Med., 2004, 204, 11-16
Address for reprints: Erol Eroglu, M. Turkes M. 5130. S., Doktorlar-Mühendisler Sitesi No. 15, 32200 Isparta, Turkey.
e-mail: heroleroglu@hotmail.com