The Effects of Vagal Stimulation on Laryngeal Vascular Resistance and Intraluminal Pressure in the Dog
NERMIN KARATURAN YELMEN, GÜLDEREN SAHIN and TÜLIN ORUÇ
University of Istanbul, Cerrahpasa Medical School, Department of Physiology, Istanbul, Turkey
In anaesthetized dogs (sodium pentobarbitone 30 mg/kg, i.v.) laryngeal vascular resistance was measured by unilateral perfusion at constant flow of the branch of the cranial superior thyroid artery that supplies the larynx. Arterial perfusion was at constant flow and inflow pressure was divided by flow to give laryngeal vascular resistance (RLV). Intraluminal laryngeal pressure (PL) and systemic arterial blood pressure (BP) were also measured. Stimulation (20 V, 20 Hz, 0.2 milliseconds) of the central end of cervical vagus caused an increase in RLV (+22.9±6.1%) and a decrease in PL (−12.1±4.4%). Stimulation (10 V, 10 Hz, 0.2 milliseconds) of the central end of the recurrent laryngeal nerve (RLN) reduced RLV (−3.4±0.8%) and PL (−7.5±4.1%). Stimulation of the peripheral end of the RLN decreased RLV (−7.1±1.9%) and increased PL (+21.6±7.7%). Stimulation of the central end of the superior laryngeal nerve (SLN) increased RLV (+17.9±3.2%) and PL (+59.8±2.7%), whereas stimulation of the peripheral end of the SLN decreased RLV (-4.8±1.6%) and PL (−4.1±2.4%). After treatment with α-adrenoreceptor antagonist phentolamine (0.5 mg/kg, i.v.), stimulation of the central end of cervical vagus nerve reduced RLV by 25% and decreased BP. Phentolamine caused a decrease in BP and reduced the magnitude of increase in RLV in response to stimulation of central end of SLN. After atropine sulphate (0.5-2.0 mg/kg, i.v.), the stimulation of both central and peripheral ends of RLN reduced RLV. The decrease in RLV during stimulation of peripheral end of SLN was reduced by atropine. Thereafter, pancuronium bromide (0.06-0.1 mg/kg, i.v.) was given and dogs were artifically ventilated. After paralyzed, stimulation of the central end of the SLN decreased RLV (+26.0±4.5%) but produced no change in PL. It is concluded that parasympathetic motor fibers in the RLN and SLN are effective for the laryngeal vascularity and non-adrenergic system may be responsible for laryngeal vasoconstriction.
keywords —— laryngeal vasculature; vagal stimulation; phentolamine; atropine
© 2004 Tohoku University Medical Press
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Tohoku J. Exp. Med., 2004, 202, 283-294
Address for reprints: Prof. Dr. GÜlderen Sahin, Istanbul University, Cerrahpaa Medical School, Department of Physiology, Istanbul, Turkey.
e-mail: sahing@istanbul.edu.tr