15-Deoxy-Δ12,14-Prostaglandin J2 Inhibits the IL-1β-Induced Expression of Granulocyte-Macrophage Colony-Stimulating Factor in BEAS-2B Bronchial Epithelial Cells
MIKA KUMAGAI, TADAATSU IMAIZUMI, KATSUHIKO SUZUKI,1 HIDEMI YOSHIDA, SHINGO TAKANASHI,2 KEN OKUMURA,2 KAZUO SUGAWARA1 and KEI SATOH
Department of Vascular Biology, Institute of Brain Science, 1Department of Hygiene, and 2Department of Internal Medicine, Hirosaki University School of Medicine, Hirosaki 036-8562
15-Deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) is an agonist for peroxisome proliferator-activated receptor-γ (PPAR-γ), which plays an important role in various biological processes including inflammatory responses. We have addressed the effect of 15d-PGJ2 on the expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) in a cell line derived from human bronchial epithelial cells (BEAS-2B). Besides being a hematopoietic growth factor, GM-CSF activates mature leukocytes and is involved in regulation of inflammatory responses. Cultures of BEAS-2B were stimulated with interleukin-1β (IL-1β), and the expressions of GM-CSF mRNA and protein were analyzed by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. IL-1β stimulated the expression of GM-CSF in BEAS-2B cells in concentration- and time-dependent manners. When the cells were pretreated with 15d-PGJ2 for 1 hour, the IL-1β-induced GM-CSF expression was inhibited in a concentration-dependent manner (2-50 μM). Ciglitazone, another agonist of PPAR-γ, did not affect the IL-1β-induced GM-CSF expression in BEAS-2B cells. A PPAR-γ antagonist, bisphenol A diglycide ether (BADGE), did not reverse the inhibitory effects of 15d-PGJ2 on GM-CSF expression. 15d-PGJ2 regulates GM-CSF expression in the bronchial epithelium, which may be mediated through a mechanism unrelated to PPAR-γ.
keywords —— 15d-PGJ2; GM-CSF; BEAS-2B; IL-1β; BADGE
© 2004 Tohoku University Medical Press
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Tohoku J. Exp. Med., 2004, 202, 69-76
Address for reprints : Dr. Tadaatsu Imaizumi, Department of Vascular Biology, Institute of Brain Science, Hirosaki University School of Medicine, 5-Zaifu-cho, Hirosaki 036-8562, Japan.
e-mail: timaizum@cc.hirosaki-u.ac.jp