Tohoku J. Exp. Med., 2002, 198 (4)

Measurement of Phosphatidylcholine Hydroperoxide in Mild
Ischemia-Reperfusion Injury in Rat Intestine

TSUYOSHI MASUKO, YUJI FUNAYAMA, HIROO NAITO, KOHEI FUKUSHIMA, CHIKASHI SHIBATA,
KEN-ICHI TAKAHASHI, HITOSHI OGAWA, TATSUYA UENO, AKIHIKO HASHIMOTO, TERUO MIYAZAWA,¹
KIYOTAKA NAKAGAWA,¹ SEIKI MATSUNO and IWAO SASAKI

Department of Surgery, Division of GI and Colorectal Surgery, Research Unit of Biological Regulation and Oncology, Tohoku University, Graduate School of Medicine, Sendai 980-8574, ¹Department of Applied Biochemistry, Tohoku University, School of Agriculture, Sendai 981-8555

In the pathogenesis of intestinal ischemia-reperfusion injury, the measurement of lipid peroxides needs to be established. Sprague-Dawley rat intestines were assessed after 30 minutes of occlusion of the superior mesenteric artery followed by reperfusion at 30, 60, 120, 180, 360 minutes. Grade of the mucosal injury, accumulation of the activated neutrophils and ICAM-1 expression were transiently increased after reperfusion. Two measuring methods of mucosal lipid peroxides using thiobarbituric acid reacting substance (TBARS) and phosphatidylcholine hydroperoxide (PCOOH) were compared. PCOOH level was significantly increased after reperfusion, while the mucosal TBARS level showed no significant change. In conclusion, lipid peroxidation could be detected with high specificity and sensitivity by measuring the mucosal phosphatidylcholine hydroperoxide level.

Keywords —— lipid peroxide; intestine; ischemia-reperfusion injury; rat

© 2002 Tohoku University Medical Press

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Tohoku J. Exp. Med., 2002, 198, 223-232

Address for reprints: Yuji Funayama, M.D., Department of Surgery, Division of GI and Colorectal Surgery, Research Unit of Biological Regulation and Oncology, Tohoku University School of Medicine, 1-1 Seiryomachi, Aoba-ku, Sendai 980-8574, Japan.

e-mail: yfunayama@surg1.med.tohoku.ac.jp