Tohoku J. Exp. Med., 2002, 198 (4)

Effect of Recombinant Basic Fibroblast Growth Factor (bFGF) on
Fibroblast-Like Cells from Human Rotator Cuff Tendon

SHU TAKAHASHI, MIZUHO NAKAJIMA, MOTO KOBAYASHI, IKUKO WAKABAYASHI,
NAOHISA MIYAKOSHI, HIROSHI MINAGAWA and EIJI ITOI

Department of Orthopedic Surgery, Akita University School of Medicine, Akita 010-8543

Rotator cuff tendon cells (RCC) derived from surgical samples showed fibroblast-like morphology. Histological staining demonstrated collagen secretion by RCC. Immunohistological findings revealed that RCC secreted type I and III collagen, but not type II collagen. In addition, the SDS-PAGE analysis suggested that RCC predominantly produced type I collagen. Basic fibroblast growth factor (bFGF) had a stimulatory effect on the proliferation of RCC dose-dependently up to 1 ng/ml. Administration of bFGF suppressed the secretion of collagens from RCC in a dose-dependent manner.

Keywords —— basic fibroblast growth factor; rotator cuff tendon; collagen; proliferation; primary culture

© 2002 Tohoku University Medical Press

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Tohoku J. Exp. Med., 2002, 198, 207-214

Address for reprints: Shu Takahashi, M.D., Department of Orthopedic Surgery, Akita University School of Medicine, 1-1-1 Hondo, Akita 010-8543, Japan.

e-mail: shu@med.akita-u.ac.jp