Lung Perfusion in Hemorrhagic Shock of Rats: The Effects of Resuscitation with Whole Blood, Saline or Hes 6%

Lung Perfusion in Hemorrhagic Shock of Rats: The Effects of Resuscitation with Whole Blood, Saline or Hes 6%

SELIM TURHANOGLU, SEDAT KAYA, ALPER KARARMAZ and AYSE DICLE TURHANOGLU

Dicle University Medical School, Diyarbakir, Turkey

This study was undertaken to determine the effects of various resuscitation regimens on lung perfusion following resuscitation from hemorrhagic shock. Fourty male Sprague-Dawley rats (250-300 g) were used. The rats were divided randomly into four groups (n=10 for each) and were sedated with intramuscular ketamine (100 mg/kg). We measured blood pressure, rectal temperature and lung perfusion using radioscintigraphy with a technetium colloid indicator. The systolic blood pressure was decreased 75% by removing blood via v. jugularis in the first three groups and group 4 was accepted as the control group, and blood volume was not diminished. Then the first three groups were resuscitated with autologous blood containing 125 units heparine/ml in group 1, saline in group 2, and hydroxyethyl starch (HES) 6% in group 3. After the correction of hypovolemia, all animals were injected 100 Bg (0.1 cc) technetium 99 m macroaggregated albumin (^99mTc MAA) via penil vein. After injection of ^99mTc MAA, 3 minutes fixed images were detected by a g camera in posterior position at 15 minutes and 5 hours. ^99mTc MAA “wash out” rate in lung was determined quantitatively at 5 hours. Compared to a control group, lung perfusion was decreased significantly in groups resuscitated with saline, and HES 6% while perfusion was restored with autologous blood. We conclude that heparinized autologous blood saved lung capillary circulation in hemorrhagic shock in rats.

Keywords —— hemorrhagic shock; lung perfusion; ^99mTc MAA; fluid resuscitation; autologous blood

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Tohoku J. Exp. Med., 2001, 195, 245-251

Address for reprints: Selim Turhanoglu, Medical School of University of Dicle, Department of Anesthesiology, Diyarbakir, Turkey.

e-mail: gaturhanoglu@superonline.com