Analysis of Plasma Nitrite/Nitrate in Human Thermal Injury

Analysis of Plasma Nitrite/Nitrate in Human Thermal Injury

DAIZOH SAITOH, AKIRA TAKASU, KUNITARO FUKUZUKA, HIROHUMI NORIO,
TOSHIHISA SAKAMOTO and YOSHIAKI OKADA

Department of Traumatology and Critical Care Medicine, National Defense
Medical College, Tokorozawa 359-8513

The aim of this study is to examine the characteristics of nitrite/nitrate (NOx), the final metabolite of nitric oxides, in plasma after burn injury. A total of 83 blood samples were collected from 19 patients on arrival, day 1, day 3, and day 5 after suffering burn injuries and from 7 non-burned volunteers. We measured the NOx levels in plasma using the Griess method, and analyzed the relationships among plasma the NOx levels, the burn-magnitude, and the blood examination data using a stepwise multivariate regression analysis. The plasma NOx levels at hospital-arrival after burns significantly exceeded those of non-burned volunteers, and the NOx levels in the plasma returned to normal range after day 1. Based on the findings of a multivariate analysis, the plasma NOx levels at admission to the hospital were not found to be related to the total burn surface area, the burn index or inhalation injury, but they were significantly related to age. Furthermore, these plasma NOx levels were also related to the platelet count, neutrophil count and blood urea nitrogen. The increase in the plasma NOx level may therefore play an important role in the pathophysiology of elderly burned patients, while the nitric oxide levels in the plasma might also play a role in inhibiting the constriction of microvascular smooth muscle in extensively burned patients.

Keywords —— burn; nitric oxides; age; neutrophil; platelet

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Tohoku J. Exp. Med., 2001, 194, 129-136

Address for reprints: Daizoh Saitoh, M.D., Department of Traumatology and Critical Care Medicine, National Defense Medical College, 3-2 Namiki, Tokorozawa 359-8513, Japan.

e-mail: ds0711@me.ndmc.ac.jp