Visualization of aggephagy and Tau fibrilization in cells by Superresolution fluorescence microscopy

Gen Matsumoto and Nozomu Mori

Department of Anatomy and Neurobiology, Nagasaki University School of Medicine

The light microscopy is a powerful and an essential tool in the research for life science, but its resolution is limitted by the diffraction of light, criating a diffraction barrir. The superresolution microscopy surpasses the diffraction barrir and enables us to observe structural details with an accuracy of nanometers schale. Here we discuss the advantages of superresolution microscopy in the observation of aggrephagy by comparison with images taken by conventional confocal or superresolution microscopes and show our recent results that the propagative Tau creates tiny fibrils in cells revieled by the superresolution structured illumination microscopy.

Address correspondence to Dr. Gen Matsumoto or Dr. Nozomu Mori, Department of Anatomy and Neurobiology, Nagasaki University School of Medicine (1-12-4 Sakamoto, Nagasaki 852-8523, Japan)